Signalling

Part:BBa_K247003:Experience

Designed by: Kaoru Yamamoto, Yoshimi Iyama   Group: iGEM09_Chiba   (2009-10-19)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K247003

User Reviews

UNIQ22e7864b03b26f8e-partinfo-00000000-QINU UNIQ22e7864b03b26f8e-partinfo-00000001-QINU The Chiba iGEM team tested this part in several conditions.

This mutant LuxR have similar speed of GFP expression of wild type LuxR but have different AHL concentration starting communication of wild type.

How the mutant LuxR was made is refer to [http://2009.igem.org/Making_delayed-LuxR_mutants this link].

Fig. 1 Time-course color development on the plate with fixed (100 nM) conc. of AHL. Left is wild type LuxR, right is mutant LuxR(Dull). Mutant LuxR obviously shows delay.


Fig. 2 Response threshold of AHL concentration. Wild type luxR is induced by 10 nM AHL. LuxR mutant containing this part show AHL threshold at about 100 nM.